FAMPAC Cells
General information
Description | Established from the primary pancreas adenocarcinoma of a 43- year-old female with a familial prediposition to pancreatic carcinoma. |
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Organism | Human |
Tissue | Pancreas |
Disease | Adenocarcinoma |
Synonyms | FamPAC, Fampac, PA-CLS-13, PA-CLS 13 |
Characteristics
Age | 43 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | FAMPAC (Cytion catalog number 300309) |
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Biosafety level | 1 |
Depositor | Dr. Schmidt |
Expression / Mutation
Protein expression | p53, point mutation (CCG (Arg) to CAC (His) |
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Antigen expression | FAMPAC cells carry a homozygous Kras mutation in codon12: GGT(Gly) >GTT(Val) |
Tumorigenic | Yes, in nude mice, adenocarcinoma |
Karyotype | 45-48, x,+3,-5,+der(5),+der(5),+der(5)add(p14),-7,+10,+2der(10)add(p15)add(q26),der(12)add(p13),der(12)add(p11),-13,-13,+der(13)add(p11),-14,der?(14),-15,i(15q),der(16)(q+),-19,-20,-21,-22,+3-5mar |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 24 to 48 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will yield in a confluent layer in about 2to3 days |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 8
D16S539: 14
D5S818: 10,11
D7S820: 11
TH01: 9
TPOX: 8
vWA: 15
D3S1358: 16,17
FGA: 32.2
D1S1656: 15
D6S1043: 12,13
D2S1338: 11
D12S391: 10,12
D19S433: 22
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HLA alleles |
A*: 03:01:01
B*: 27:05:02
C*: 15:02:01
DRB1*: 12:01:01
DQA1*: 05:05:01
DQB1*: 03:01:01
DPB1*: 03.01:01
E: 01:01:01
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