E11 Cells
$800.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | The E11 cell line has been meticulously cloned from the outgrowth of glomeruli, which were isolated from H-2kb-tsA58 transgenic mice. These transgenic mice carry a temperature-sensitive variant of the SV40 large T antigen, under the precise control of the IFN-g-inducible H-2kb promoter. This intricate genetic arrangement ensures the controlled development and sustained viability of the E11 cell line. One of the most remarkable attributes of the E11 cell line is its exceptional stability. Researchers have successfully propagated these cells for more than 40 passages without any observed phenotypic alterations. This enduring stability has facilitated rigorous experimentation and in-depth research, making the E11 cell line an ideal candidate for studies in podocyte biology. These cells exhibit stable expression of nephrin, a pivotal protein closely associated with podocytes. Furthermore, they display a remarkable capacity for forming abundant cell-cell contacts, a critical characteristic for mimicking the behavior of podocytes in their native environment. E11 cells not only stably express nephrin but also demonstrate the presence of a spectrum of other essential podocyte proteins. This comprehensive protein expression profile includes NEHP1, FAT, P-cadherin, podocin, CD2AP, ZO-1 (?-isoform), Lmx1b, podoplanin, synaptopodin, cortactin, and vimentin. |
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Organism | Mouse |
Tissue | Kidney |
Characteristics
Age | Adult |
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Gender | Unspecified |
Cell type | Podocyte |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | E11 (Cytion catalog number 400425) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | WT1, Lmx1b, nephrin, NEPHI, FAT, P-cadherin, CD2AP, ZO-I, podocalyxin, podoplanin, synpo, podocin, TRPC6 and GAPDH. |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 or 1:5 (at 33 degree Celsius) is recommended Under differentiation conditions, ie incubation of non to confluent cultures at 38 degree Celsius, cell proliferation ceases within the first two weeks and stops after about four weeks |
Seeding density | Inocculate T75 cell culture flasks with 1x 10^4 cells/cm^2 for the proliferation process. Keep the cells at 33 degree Celsius / 5% CO2, until the flask is about 75% confluent. |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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