DU-145 Cells
Investigating androgen receptor influence in the human prostate cancer cell line DU-145
Description | DU145 is a human prostate cancer cell with an epithelial morphology commonly used in prostate cancer research. The cell line was established from the brain of a 69-year-old male with prostate cancer. They express androgen receptors and are considered tumorigenic with moderate metastatic potential, forming adenocarcinoma (grade II) consistent with prostatic primary when injected into nude mice. In terms of karyotype, DU145 cells are hypotriploid and have several marker chromosomes, including t(11q12q), del(11)(q23), 16q+, del(9)(p11), del(1)(p32), among others. They express several isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3. However, the cells do not express the prostate antigen. DU145 cells are weakly positive for acid phosphatase and capable of forming colonies in soft agar. The presence of microvilli, tonofilaments, desmosomes, any mitochondria, well-developed Golgi, and heterogenous lysosomes was reported by ultrastructural analyses. DU145 cells have a doubling time of approximately 30-40 hours and are suitable transfection hosts. DU145 cells are a valuable tool in the therapeutic research of prostate cancer. Along with PC3 and LNCaP cell lines, DU145 is a standard prostate cancer cell line used in medicinal research. Along with PC-3 cells, DU-145 cells express androgen receptor proteins. However, when treated with an androgen ligand, the cells did not show stimulation of the activity of an AR-responsive reporter gene. Therefore, these cells are considered to be androgen non-responsive. |
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Organism | Human |
Tissue | Prostate |
Disease | Carcinoma |
Metastatic site | Brain |
Synonyms | DU145, Du-145, DU 145, DU_145, DU.145, Duke University 145 |
Features
Age | 69 years |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Specification
Citation | DU-145 (Cytion catalog number 300168) |
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Biosafety level | 1 |
Genotype of the human prostate cancer cell line DU-145
Antigen expression | Blood Type O, Rh+ |
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Isoenzymes | Me-2, 1-2, PGM3, 2, PGM1, 1, ES-D, 1, AK-1, 1, G6PD, B, GLO-1, 2, Phenotype Frequency Product: 0.0041 |
Tumorigenic | Forms adenocarcinoma (grade II) consistent with prostatic primary |
Karyotype | (P75) hypotriploid to tetraploid with abnormalities including breaks, dicentrics, minutes and large telocentric marker |
Handling DU145 cells
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Genetic profile
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,11,12
D13S317: 12,13,14,15
D16S539: 11,13
D5S818: 10,12,13
D7S820: 7,10,11
TPOX: 11
vWA: 17,18
D3S1358: 16,17
D21S11: 30,33
D18S51: 12
Penta E: 12,14
Penta D: 9,13
D8S1179: 13,14
FGA: 22
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HLA alleles |
A*: 03:21N, 33:03:01
B*: 50:01:01, 57:01:01
C*: 06:02:01
DRB1*: 01:01:01, 07:01:01
DQA1*: 01:01:01, 02:01:01
DQB1*: 03:03:02, 05:01:01
DPB1*: 04:01:01
E: 01:01:01, 01:09
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