DSL-6A-C1 Cells
General information
Description | DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma. The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat(DSL-101-79) that was given azaserine intraperitoneally. The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture. The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting. The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator (CFTR). |
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Organism | Rat |
Tissue | Pancreas |
Disease | Carcinoma, azaserine induced |
Metastatic site | Ductal |
Synonyms | DSL-6A/C1, DSL6A/C1 |
Characteristics
Age | 2 years |
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Gender | Male |
Morphology | Epithelial-like |
Cell type | Acinar cells |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | DSL-6A-C1 (Cytion catalog number 500166) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in Lewis rats the cells produce solid tumors composed of ductlike structures surrounded by dense fibrous tissue |
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Handling
Culture Medium | Waymouth medium (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance) |
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Medium supplements | Supplement the medium with 10% FBS, 2.0 mM L-glutamine |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D1Wox31: 104
Rat_D2Wox37: 156
Rat_D19Wox11: 232
Rat_D10Wox8: 266
Rat_D4Wox7: 157
Rat_D2Wox27: 207
Rat_D5Rat33: 122
Rat_D10Wox11: 171
Rat_D1Wox23: 210
Rat_D12Wox1: 406
Rat_D6Wox2: 104
Rat_D8Wox7: 182
Rat_D6Cebr1: 239
SRY: x,Y
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