DMS-79 Cells
General information
Description | The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung. The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy. The cells express HLA class I and class II antigens. |
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Organism | Human |
Tissue | Lung |
Disease | Carcinoma, azaserine induced |
Metastatic site | Pleural effusion |
Synonyms | DMS 79, DMS79 |
Characteristics
Age | 65 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Aggregates in suspension |
Identifiers / Biosafety / Citation
Citation | DMS-79 (Cytion catalog number 300164) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Epidermal growth factor (EGF) |
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Antigen expression | Leu 7, My23, Class 1 HLA, Class 2 HLA |
Oncogenes | c-myc +, N-myc +, c-raf-1 +, Ha-ras +, Ki-ras +, N-ras +, v-fes -, v-fms - |
Tumorigenic | Yes, in nude mice |
Products | Adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, calcitonin, corticotropin, beta endorphin, 17 beta estradiol, lipotropin, oxytocin - neurophysin (OT-NP), parathormone, somatostatin-like immunoreactivity (SRIF) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 96 hours |
Subculturing | Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Subculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate at 37 degree Celsius for 10minutes. Collect the cells following centrifugation, resuspend in fresh cell culture medium and count. Start cultures at 2-4 x 10^4 cells/ml. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 2 to 4 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 10
D7S820: 9,11
TH01: 8
TPOX: 8
vWA: 18
D3S1358: 8
D21S11: 30
D18S51: 14,17
Penta E: 7
Penta D: 11,13
D8S1179: 12,14
FGA: 21
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HLA alleles |
A*: 01:01:01, 02:01:01
B*: 08:01:01, 35:01:01
C*: 04:01:01, 07:01:01
DRB1*: 11:01:01, 14:01:01
DQA1*: 01:04:01, 05:05:01
DQB1*: 03:01:01, 05:03:01
DPB1*: 03:01:01, 10:01:01
E: 01:01, 01:03
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