DLD-1 Cells
Insights on DLD-1 cells
Description | The DLD-1 cell line, derived from the colorectal adenocarcinoma of a 58-year-old male patient, is recognized for its relevance in cancer research, particularly in the study of colorectal carcinomas. DLD- 1 offers a unique model for investigating the complexities of cancer cell biology, drug resistance mechanisms, and the role of genetic and chromosomal alterations in cancer progression. DLD-1 human colon carcinoma cells express SIRT1 (sirtuin 1), a protein involved in the deacetylation of transcription factors, which plays a significant role in colorectal adenocarcinoma's pathophysiology. The presence of SIRT1 and its implications in cancer biology, provide a valuable framework for exploring the molecular mechanisms underlying cancer progression. DLD-1 cells have various chromosome changes and show aspects of chromosome stability. These features provide insights into the cellular metabolic status of cancer cells and their adaptation to the tumor microenvironment. The cell line's utility is further extended by its application in quantitative proteomic analyses, allowing for a deeper understanding of the protein expression profiles and metabolic pathways active in colorectal adenocarcinoma. The cell line's relevance to cancer drug resistance studies is underscored by its application in screening and evaluating the efficacy of various compounds, including those affecting metabolic pathways and epigenetic regulators. In summary, the DLD-1 cell line serves as a crucial tool in the landscape of human cancer cell lines, particularly for studying human colon cancer and carcinoma. |
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Organism | Human |
Tissue | Colon |
Disease | Adenocarcinoma |
Synonyms | DLD 1, DLD1, CoCL3 |
Aspects of the colorectal adenocarcinoma cell line DLD-1
Age | 67 years |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Documentations
Citation | DLD-1 (Cytion catalog number 300220) |
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Biosafety level | 1 |
Genotype
Protein expression | Keratin |
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Tumorigenic | In nude mice |
Viruses | Reverse Transcriptase negative |
Products | Carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days, alkaline phosphatase |
Karyotype | 2n = 46 |
Handling DLD-1 colorectal adenocarcinoma SIRT1 cells
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 15 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 1 to 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
TH01: 7,9.3
TPOX: 8,11
vWA: 18,19
D3S1358: 17
D21S11: 29,32.2
D18S51: 11,17
Penta E: 7,14
Penta D: 9,14
D8S1179: 15
FGA: 22
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