DF-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$650.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305016

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	DF-1 cells are a continuous cell line derived from chicken embryonic fibroblasts, specifically from East Lansing Line (ELL-0) chickens. The cell line is noted for its lack of endogenous avian leukosis virus, which is a common contaminant in many other chicken cell lines. This characteristic makes DF-1 cells particularly valuable for virology research, especially in studies involving the propagation and genetic manipulation of viruses that infect birds, such as avian influenza and Marek's disease virus. In addition to their use in virology, DF-1 cells are utilized in various areas of cellular and molecular biology research. They have a robust growth rate and a fibroblast-like morphology, making them suitable for in vitro experiments that require a stable avian cell environment. These cells have been instrumental in gene expression studies, especially concerning the effects of viral and other genetic elements in avian species. The genetic stability and susceptibility to transfection also make DF-1 an excellent model for studying gene function and regulation in a controlled environment.
Organism	Chicken
Tissue	Embryo
Synonyms	DF1, UMNSAH-DF-1, UMNSAH-DF 1, UMNSAH-DF1, UMNSAH/DF1, UMNSAH/DF#1, Douglas Foster-1, UMNSAH/DF-1

Age	10 days gestation
Morphology	Fibroblast
Growth properties	Adherent

Citation	DF-1 (Cytion catalog number 305016)
Biosafety level	1
NCBI_TaxID	9031
CellosaurusAccession	CVCL_0570

Tumorigenic	No

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305016-121222	Certificate of Analysis	23. May. 2025	305016
305016-181024	Certificate of Analysis	23. May. 2025	305016
305016-091123	Certificate of Analysis	23. May. 2025	305016

DF-1 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)