DC2.4 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305515

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The DC2.4 cell line is an immortalized mouse dendritic cell line that originates from bone marrow. It is commonly used to study dendritic cell (DC) biology, immune responses, and the development of immunotherapies. DC2.4 cells are characterized by their role as antigen-presenting cells (APCs) and are known to express typical surface markers of dendritic cells, such as CD11c and MHC class I molecules. However, they exhibit an immature phenotype under standard culture conditions, with low expression of MHC class II and costimulatory molecules like CD40 and CD80. This makes them useful for investigating the mechanisms and stimuli required for DC maturation and their subsequent immune functions. Studies have shown that specific stimuli can induce maturation of DC2.4 cells. Notably, exposure to interferon-gamma (IFN-γ) leads to significant upregulation of MHC class II, CD40, CD80, and CCR7, as well as increased cytokine secretion, including IL-6, IL-12, and TNF-α. IFN-γ-matured DC2.4 cells have been demonstrated to effectively activate CD8+ cytotoxic T cells both in vitro and in vivo, enhancing antitumor immunity. For instance, IFN-γ-treated, antigen-pulsed DC2.4 cells have been shown to induce robust CD8+ T cell responses and provide protective antitumor effects in mouse models. This highlights the cell line's utility in cancer immunotherapy research and vaccine development. Additionally, DC2.4 cells have been employed to study host-pathogen interactions, as their response to various immune challenges can mimic aspects of the innate immune system's activation. The analysis of exosomal miRNA profiles from DC2.4 cells, especially when infected with pathogens like Toxoplasma gondii, has provided insights into the molecular mechanisms underlying dendritic cell signaling and immune communication. The differential expression of exosomal miRNAs in response to infection suggests potential roles in modulating host immunity and highlights the utility of DC2.4 in exosome and RNA-based immune research.
Organism	Mouse
Tissue	Bone marrow
Synonyms	DC 2.4

Breed/Subspecies	C57BL/6
Age	Unspecified
Gender	Unspecified
Cell type	Dendritic cell
Growth properties	Adherent

Citation	DC2.4 (Cytion catalog number 305515)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_J409
GMO Status	GMO-S1: This murine dendritic cell line (DC2.4) contains retroviral constructs encoding murine GM-CSF, v-myc and v-raf introduced by transduction, supporting transformation and growth. The inserts are stably present in the dendritic cell-derived line. This classification applies only within Germany and may differ elsewhere.

Viruses	Transformant: Recombinant retrovirus J2

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS, 1% NEAA and 10mM HEPES
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305515-111124	Certificate of Analysis	23. May. 2025	305515
305515-240226	Certificate of Analysis	27. Mar. 2026	305515
305515-050625	Certificate of Analysis	21. Jul. 2025	305515

DC2.4 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)