DAN-G Cells
General information
Description | The line was derived from nude mouse xenografts initiated with cells from the tumor of a patient with cancer of the pancreas. |
---|---|
Organism | Human |
Tissue | Pancreas |
Disease | Adenocarcinoma |
Synonyms | Dan-G, DanG, DANG |
Characteristics
Age | 68 years |
---|---|
Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | DAN-G (Cytion catalog number 300162) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 negative |
---|---|
Tumorigenic | Yes, in nude mice |
Mutational profile | DAN-G cells carry a homozygous Kras mutation in codon12: GGT(Gly) >GTT(Val) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 33 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | 3 to 4 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 13
D13S317: 8
D16S539: 8,11
D5S818: 12,13
D7S820: 10,13
TH01: 9.3
TPOX: 10
vWA: 16,18
D3S1358: 16
D21S11: 29,31.2
D18S51: 16
Penta E: 7
Penta D: 9,13
D8S1179: 10,11
FGA: 20
D1S1656: 12,17
D6S1043: 12
D2S1338: 17,18
D12S391: 17,20
D19S433: 13,14
|
HLA alleles |
A*: 02:01:01
B*: 07:02:01, 13:02:01
C*: 06:02:01, 07:02:01
DRB1*: 07:01:01, 15:01:01
DQA1*: 01:02:01, 02:01:01
DQB1*: 02:02:01, 06:02:01
DPB1*: 04:01:01, 17:01:01
E: 01:03:02
|