Colo-205 Cells
General information
Description | COLO-205 cells express a 36,000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. |
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Organism | Human |
Tissue | Colon, Dukes' type D |
Disease | Colorectal adenocarcinoma |
Metastatic site | Ascites |
Synonyms | Colo 205, CoLo 205, COLO-205, COLO 205, COLO.205, Colo205, COLO205, Co 205, Colorado 205 |
Characteristics
Age | 70 years |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent/suspension, loosely attached |
Identifiers / Biosafety / Citation
Citation | Colo-205 (Cytion catalog number 300380) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | CSAp- (Centriole and Spindle?Associated protein) |
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Antigen expression | The cells are positive for keratin by immunoperoxidase staining. |
Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1-2, 6PGD, A, ES-D, 1-2, PEP-D, 1 |
Tumorigenic | Yes, in nude mice |
Reverse transcriptase | Negative |
Products | Carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/106 cells/10 days, keratin, interleukin 10 (IL-10, interleukin-10) |
Ploidy status | Aneuploid |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 20 to 25 hours |
Subculturing | Collect suspension cells in a 15 ml tube and carefully rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes, then centrifuge the cells growing in suspension and the adherent cells together. Carefully resuspend the cells and dispense into new flasks which contain fresh medium. |
Split ratio | Subcultivation ratios of 1:2 to 1:10 are possible when all cells are pooled (suspended cells plus cells recovered after using Accutase) |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
TH01: 8,9
TPOX: 11
vWA: 15
D3S1358: 16
D21S11: 30.2,33.2
D18S51: 18
Penta E: 13,15
Penta D: 9,11
D8S1179: 9,14
FGA: 21,23
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HLA alleles |
A*: 01:01:01, 02:01:01
B*: 07:02:01, 08:01:01
C*: 07:01:01, 07:02:01
DRB1*: 04:01:01, 13:01:01
DQA1*: 01:03:01
DQB1*: 06:03:01
DPB1*: 04:01:01
E: 01:01:01, 01:03
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