Chang Liver (HeLa) Cells
General information
Description | Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. The cells are positive for keratin by immunoperoxidase staining. |
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Organism | Human |
Tissue | Liver |
Disease | Adenocarcinoma |
Synonyms | Chang-liver, Chang Cells, Chang, CHL |
Characteristics
Age | 30 years |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Chang Liver (HeLa) (Cytion catalog number 300139) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, A |
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Tumorigenic | Yes, in Syrian hamsters |
Viruses | Tested MHV (mouse hepatitis virus) negative |
Virus susceptibility | Poliovirus 1, 2, 3, adenovirus 3, vesicular stomatitis (Indiana) |
Reverse transcriptase | negative |
Products | keratin |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 12
D7S820: 8,12
TH01: 7
TPOX: 8,12
vWA: 16,18
D3S1358: 15,18
D21S11: 27,28
D18S51: 16
Penta E: 7,17
Penta D: 8,15
D8S1179: 12,13
FGA: 21
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HLA alleles |
A*: 68:02:01
B*: 15:03:01
C*: 12:03:01
DRB1*: 01:02:01
DQA1*: 01:01:02
DQB1*: 05:01:01
DPB1*: 01:01:01
E: 01:03:02
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