Capan-1 Cells
Basic details about CaPan-1 cell lines
Description | The cells express the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete gastric type mucin. |
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Organism | Human |
Tissue | Pancreas |
Disease | Ductal adenocarcinoma |
Metastatic site | Liver |
Synonyms | CaPan-1, CAPAN-1, Capan 1, CAPAN 1, Capan1, CAPAN1 |
Properties of the pancreatic adenocarcinoma cell line Capan-1
Age | 40 years |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Specification
Citation | Capan-1 (Cytion catalog number 300143) |
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Biosafety level | 1 |
Genomics of Capan-1 pancreatic cancer cells
Protein expression | p53 negative |
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Antigen expression | Blood Type A, Rh+ |
Isoenzymes | Me-2, 1, PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1, G6PD, B, GLO-1, 1-2, Phenotype Frequency Product: 0.0311 |
Tumorigenic | Form adenocarcinoma consistent with pancreatic duct carcinoma |
Products | Mucin |
Mutational profile | Capan-1 cells carry a homozygous Kras mutation in codon12: GGT(Gly) >GTT(Val) |
Karyotype | (P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker |
Cell culture practices
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 60 to 80 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 will result in a 90% confluent monolayer in about 7 days |
Fluid renewal | Every 3 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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CaPan-1 cell quality verification
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11
D13S317: 9
D16S539: 13,14
D5S818: 11
D7S820: 10,11
TH01: 6
TPOX: 8,11
vWA: 16
D3S1358: 15
D21S11: 28,30
D18S51: 12
Penta E: 10,12
Penta D: 9,13
D8S1179: 14,16
FGA: 24
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HLA alleles |
A*: 01:01:01, 30:01:01
B*: 13:02:01, 57:01:01
C*: 06:02:01
DRB1*: 07:01:01, 13:05:01
DQA1*: 02:01:01, 05:05:01
DQB1*: 02:02:01, 03:01:01
DPB1*: 03:01:01G, 04:01:01G
E: 01:01:01
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