Calu-6 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300135

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The Calu-6 cell line is a human non-small cell lung carcinoma (NSCLC) cell line derived from the pleural effusion of a 61-year-old male patient. Established in 1975, this cell line has been a critical model in lung cancer research. Calu-6 cells exhibit a distinct epithelial morphology and have been used extensively to study the biology of lung cancer, including mechanisms of metastasis, drug resistance, and the tumor microenvironment. These cells are particularly noted for their ability to form tumors in xenograft models, which makes them highly valuable for in vivo studies of tumor growth and response to therapeutics. Calu-6 is characterized by a high level of KRAS mutation, common in NSCLC, and provides a relevant model for studying the role of this oncogene in lung cancer. The cell line also displays several cytogenetic anomalies typical of cancer cells, such as complex karyotypes and aneuploidy, which contribute to its use in genetic studies. Research utilizing the Calu-6 cell line has helped in understanding the cellular mechanisms of lung cancer and in the development of therapeutic strategies. Its robust growth in culture and the ability to mimic clinical aspects of lung cancer make it an indispensable resource in oncological research.
Organism	Human
Tissue	Lung
Disease	Adenocarcinoma
Metastatic site	Pleural effusion
Synonyms	CaLu-6, CALU-6, Calu.6, Calu 6, Calu6, CALU6, CaLu-06

Age	61 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent

Citation	Calu-6 (Cytion catalog number 300135)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0236

Protein expression	P53 negative
Isoenzymes	Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0031
Tumorigenic	Yes, in nude mice. Forms poorly differentiated carcinoma
Mutational profile	CaLu-6 cells carry a mutation in KRAS codon 61, c.181C>A p.(Gln61Lys). NRAS of BRAF mutation was not detected.
Karyotype	The stemline chromosome number is hypotriploid and the 2S component occurred at 5.8%. Modal chromosome number is 59. Fourteen marker chromosomes (constitutive) were common to most S metaphases. No Y chromosome was detected in the QM stained preparation.

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	2 x 10⁴ cells/cm² will result in a 90% confluent monolayer in about 4 days
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300135-040425	Certificate of Analysis	23. May. 2025	300135
300135-090124	Certificate of Analysis	23. May. 2025	300135
300135-412	Certificate of Analysis	23. May. 2025	300135

Calu-6 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)