Caki-1 Cells
General information
Organism | Human |
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Tissue | Kidney |
Disease | Clear cell carcinoma |
Synonyms | CAKI-1, CaKi-1, caki-1, CAKI.1, CAKI 1, CAKI1, Caki1 |
Characteristics
Age | 49 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | Caki-1 (Cytion catalog number 300149) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12
D5S818: 11,12
D7S820: 8,12
TH01: 6,8
TPOX: 8,11
vWA: 15,17
D3S1358: 17
D21S11: 28,30
D18S51: 14
Penta E: 22,23
Penta D: 11,12
D8S1179: 12,14
FGA: 26
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HLA alleles |
A*: 23:01:01, 24:02:01
B*: 35:02:01, 44:03:01
C*: 04:01:01, 04:63
DRB1*: 07:01:01, 11:04:01
DQA1*: 02:01:01, 05:05:01
DQB1*: 02:02:01, 03:01:01
DPB1*: 02:01:02, 10:01:01
E: 01:01:01, 01:03:01
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