CaSki Cells
General information
Description | The line was established using cells from a metastasis in the small bowel mesentery. The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. |
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Organism | Human |
Tissue | Cervix |
Disease | Carcinoma |
Metastatic site | Cervix |
Synonyms | Ca-Ski, Ca Ski, Caski, CASKI |
Characteristics
Age | 40 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Cell type | Epidermoid |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | CaSki (Cytion catalog number 300145) |
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Biosafety level | 2 |
Expression / Mutation
Isoenzymes | G6PD, B |
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Products | beta subunit of hCG, tumor associated antigen |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will result in a confluent monolayer within 3to4 days. |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 8,11
TH01: 7
TPOX: 8
vWA: 17
D3S1358: 15
D21S11: 30
D18S51: 17
D8S1179: 15
FGA: 21
D2S1338: 21
D19S433: 15,16
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HLA alleles |
A*: 02:01:01, 03:01:01
B*: 07:02:01, 37:01:01
C*: 07:02:01
DRB1*: 08:01:01G, 15:01:01G
DQA1*: 01:02:01, 04:02
DQB1*: 04:02:01, 06:02:01
DPB1*: 04:01:01
E: 01:03:02
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