COLO-320 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305315

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	COLO-320 is a human colorectal adenocarcinoma cell line derived from a metastatic tumor. It is extensively used in research to study colorectal cancer biology, drug resistance mechanisms, and the effects of anticancer agents. The COLO-320 cells are characterized by the overexpression of various oncogenes, such as c-myc, which is known to drive cell proliferation. This cell line is also a model for investigating the role of epigenetic modifications in cancer progression, as it exhibits frequent DNA methylation and histone acetylation abnormalities that influence the expression of key tumor suppressor genes and cell cycle regulators. Research on COLO-320 has revealed that the methylation status of the CDKN2A (p16) promoter is a critical factor in the regulation of this tumor suppressor gene, which is responsible for inhibiting the G1/S phase transition in the cell cycle. Treatment with DNA methyltransferase inhibitors like 5-aza-2’-deoxycytidine (5-aza-dC) has been shown to demethylate the CDKN2A promoter, restoring gene expression and potentially inducing growth arrest. Furthermore, histone deacetylase inhibitors (HDACis) such as trichostatin A (TSA) can modulate histone acetylation levels, affecting the expression of other genes, including p21WAF1. However, studies have indicated that histone acetylation specifically influences p21WAF1 without significantly impacting CDKN2A-associated histones, suggesting differential epigenetic regulation. Another area of research focuses on COLO-320's role in multidrug resistance (MDR). The cell line has been used to evaluate the efficacy of various MDR modulators. Phenothiazine derivatives, for instance, have demonstrated the ability to inhibit P-glycoprotein (P-gp) activity, a protein often overexpressed in COLO-320 cells that pumps out chemotherapeutic drugs, thereby reducing their intracellular concentration and efficacy. Studies have utilized flow cytometry with rhodamine 123 efflux assays to quantify the effectiveness of these compounds in reversing MDR, providing insights into potential strategies to overcome drug resistance in colorectal cancer.
Organism	Human
Tissue	Colon
Disease	Adenocarcinoma
Synonyms	Colo-320, Colo 320, COLO #320, COLO320, Colo320, CoLo320, Co320, COLO 320F, Colorado 320, COLO 320

Age	55 years
Gender	Female
Ethnicity	Caucasian
Growth properties	Adherent/suspension

Citation	COLO-320 (Cytion catalog number 305315)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1989

Mutational profile	Mutation: TP53, p.Arg248Trp (c.742C>T)

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS, 2,5 g/L glucose, 10 mM HEPES
Dissociation Reagent	Accutase
Seeding density	2 - 4 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305315-080526	Certificate of Analysis	24. Jun. 2026	305315

COLO-320 Cells

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Certificate of Analysis (CoA)