CLS-ACI-1 Cells
General information
Description | The solid Mammary carcinoma was induced by oral administration of 20 mg DMBA (7,12-dimethylbenzo[a]anthracene) per Kg weight. In 1998, the continuous cell line CLS-ACI-1 was established as in vitro culture from the solid tumor tissue by CLS Cell Lines Service. |
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Organism | Rat |
Tissue | Breast |
Disease | Adenocarcinoma |
Synonyms | CLS-ACI-I |
Characteristics
Age | 3 months |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | CLS-ACI-1 (Cytion catalog number 500459) |
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Biosafety level | 1 |
Expression / Mutation
Oncogenes | Overexpression of Mycn gene. |
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Tumorigenic | Yes, in nude mice, ACI-rat |
Karyotype | Near triploid. 88.4% showing 51-69 chromosomes, 5% 38-50 chromosomes, 6.6% near tetraploid or higher ploidy level. |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Split ratio | A ratio of 1:3 to 1:5 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 will yield in a confluent layer in about 6 to 7 days |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D1Wox31: 100
Rat_D2Wox37: 156
Rat_D19Wox11: 228
Rat_D10Wox8: 266,270
Rat_D4Wox7: 141,145
Rat_D2Wox27: 223
Rat_D5Rat33: 116,120,122
Rat_D10Wox11: 156,159
Rat_D1Wox23: 226,230
Rat_D12Wox1: 410
Rat_D6Wox2: 100,112,120
Rat_D8Wox7: 161,182
Rat_D6Cebr1: 239,241
SRY: x,x
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