CLS-54 Cells
General information
Description | Established in vitro from the primary lung carcinoma of a 65 year-old man in 1998 by CLS. |
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Organism | Human |
Tissue | Lung |
Disease | Adenocarcinoma |
Characteristics
Age | 65 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | CLS-54 (Cytion catalog number 300227) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 11
D16S539: 12,13
D5S818: 13
D7S820: 10,11
TH01: 6,9.3
TPOX: 8,9
vWA: 14,17
D3S1358: 18
D21S11: 30,31.2
D18S51: 11,17,18
Penta E: 12,15
Penta D: 9
D8S1179: 11
FGA: 20
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HLA alleles |
C*: 03:04:01, 04:01:01
DRB1*: 04:02:01, 07:01:01
DQA1*: 02:01:01, 03:01:01
DQB1*: 02:02:01, 03:02:01
DPB1*: 04:01:01, 11:01:01
E: 01:01:01, 01:03:01
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