CLS-354 Cells
General information
Description | CLS-354 cells are a remarkable cell line cultivated in vitro since 1998. These cells originated from the primary squamous carcinoma of a 51-year-old Caucasian male, making them a valuable resource for researchers in biological science. CLS-354 cells were obtained from the oral cavity, specifically the mouth, providing a unique perspective for studying various aspects of oral cancer and related diseases. This cell line offers an opportunity to delve into the intricate mechanisms and characteristics of squamous cell carcinoma, a common cancer affecting the oral cavity. By utilizing CLS-354 cells, researchers can investigate the molecular pathways, genetic alterations, and phenotypic changes associated with squamous cell carcinoma. This cell line is a reliable model for studying the disease's progression, metastasis, and potential therapeutic interventions. Due to their establishment from a primary tumour, CLS-354 cells retain essential features and characteristics of squamous carcinoma, making them an invaluable tool for conducting experiments and investigations in vitro. Researchers can explore these cells' growth kinetics, cellular morphology, and behaviour to gain deeper insights into the pathology of squamous cell carcinoma. With a well-documented history dating back to their creation in 1998, CLS-354 cells have been extensively utilized in numerous studies and research projects. Their consistent and reproducible behaviour allows for accurate and reliable experimental results, contributing to advancing knowledge in oral cancer research. CLS-354 cells provide researchers with a powerful tool to investigate the intricacies of squamous cell carcinoma in the oral cavity. Their origin from a primary tumour and their long-established in vitro culture ensure they possess the critical characteristics required for insightful and reliable biological studies. With CLS-354 cells, researchers can further unravel the complexities of oral cancer and pave the way for novel therapeutic strategies and interventions. |
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Organism | Human |
Tissue | Oral cavity |
Disease | Squamous cell carcinoma |
Synonyms | xF354, xF 354 |
Characteristics
Age | 51 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | CLS-354 (Cytion catalog number 300152) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Reverse transcriptase | negative |
Products | keratin |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will yield in a confluent layer in about 6to7 days |
Fluid renewal | Every 2 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,12
D13S317: 9,13
D16S539: 9,11
D5S818: 9,12
D7S820: 7,9
TH01: 9,9.3
TPOX: 8
vWA: 15,17
D3S1358: 16
D21S11: 28
D18S51: 15
Penta E: 10,14
Penta D: 13
D8S1179: 12,14
FGA: 21,23
D1S1656: 12
D6S1043: 11
D2S1338: 25
D12S391: 17,21
D19S433: 15,15.2
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HLA alleles |
A*: 01:01:01, 24:02:01
B*: 08:01:01, 18:01:01
C*: 07:01:01, 12:03:01
DRB1*: 03:01:01, 11:03:01
DQA1*: 05:01:01, 05:05:01
DQB1*: 02:01:01, 03:01:01
DPB1*: 01:01:01, 04:02:01
E: 01:01:01, 01:03
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