CLS-145 Cells
General information
Description | The CLS-145 cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. MAP-Test negative, PAS positive. |
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Organism | Human |
Tissue | Stomach |
Disease | Gastric papillary adenocarcinoma |
Synonyms | CLS145 |
Characteristics
Age | 70 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | CLS-145 (Cytion catalog number 300180) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 positive |
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Tumorigenic | Yes, in nude mice (Rhythm 20-30 days) |
Karyotype | Modal number: Mode of 109 and 110 chromosomes |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 36 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11
D13S317: 11,12
D16S539: 11,12
D5S818: 13
D7S820: 11
TH01: 7
TPOX: 11
vWA: 16,18
D3S1358: 17
D21S11: 30.2,32.2
D18S51: 17
Penta E: 12,13,14
Penta D: 9,10
D8S1179: 11,14
FGA: 22
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HLA alleles |
A*: 01:01:01
B*: 35:03:01
C*: 04:01:01
DRB1*: 01:01:01, 13:01:01
DQA1*: 01:01:01, 01:03:01
DQB1*: 05:01:01, 06:03:01
DPB1*: 04:01:01G, 06:01:01G
E: 01:01:01
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