CERV-215 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300292

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The CERV-215 cell line, established by Dr. Bodgen at the Mason Research Institute, originates from a primary xenotransplant termed MRI-H215, which has been adapted for in vivo transplantation. This cell line represents an aggressive form of epidermoid carcinoma, categorized as invasive, large cell, non-keratinizing, and poorly differentiated. The Cerv-215 cell line, is a pivotal resource for cancer research, especially in the study of genetic alterations and their roles in cervical carcinogenesis. This cell line is characterized by unique genetic modifications in the Smad4 gene, where specific exons are replaced by sequences from other genomic regions, leading to the expression of truncated and likely non-functional Smad4 proteins. These alterations provide insights into the cell line's oncogenic properties and the molecular mechanisms underlying cervical cancer. Notably, MRI-215 is HPV45 positive, yet its Smad4 gene alterations are independent of HPV integration, suggesting a complex interplay of genetic factors contributing to cancer development beyond viral influences. This cell line serves as an invaluable tool for researchers focusing on the genetic aspects of cancer, the role of Smad4 in tumor progression, and the interaction between human papillomavirus and host cellular mechanisms. MRI-H215 offers a unique platform for exploring the intricacies of cervical cancer at the molecular level, making it an essential component of cancer research laboratories aiming to uncover new therapeutic targets and understand the genetic basis of tumorigenesis.
Organism	Human
Tissue	Cervix
Disease	Carcinoma
Synonyms	Cerv-215, MRI-H-215, MRI-H215

Age	39 years
Gender	Female
Ethnicity	African
Morphology	Epithelial-like
Cell type	Epidermoid
Growth properties	Adherent

Citation	CERV-215 (Cytion catalog number 300292)
Biosafety level	2
NCBI_TaxID	9606
CellosaurusAccession	CVCL_5722

Tumorigenic	Yes, in nude mice
Viruses	HPV-16 negative
Products	Cytokeratine 8, 18, Vimentin

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm² is recommended
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300292-150124	Certificate of Analysis	18. Aug. 2025	300292
300292-150123	Certificate of Analysis	23. May. 2025	300292

CERV-215 Cells

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)