CERV-186 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300290

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The CERV-186 cell line, derived in vitro from the xenotransplant of cervical carcinoma MRI-H-186, serves as a biological model for invasive, large-cell, non-keratinizing squamous cell carcinoma. This cell line was established and adapted for in vivo transplantation under the direction of Dr. Bodgen at the Mason Research Institute. Characterized by its genomic properties, MRI-H186 contains approximately 26 integrated copies of both full-length and truncated forms of the HPV16 genome, which significantly influence its transcriptomic profile. MRI-H186 cells are distinguished by their robust expression of both full-length and truncated early HPV16 transcripts, notably displaying high levels of E5 full-length (fl) RNA. This transcriptional signature is notably distinct from that observed in other cervical carcinoma cell lines such as CaSki and MRI-H196. Additionally, the transcriptional activity of MRI-H186, in terms of the expression of various other transcripts, shows a close alignment with the patterns observed in the HPK-IA and C3 cell lines, indicating a similar transcriptional behavior across these models. The presence of both full-length and truncated HPV16 genomic integrations in MRI-H186 cells is a key factor in their vigorous expression of early viral transcripts, particularly underscored by the significant expression of E5 fl RNA. This intense transcriptional activity culminates at the early polyadenylation signal, highlighting the unique transcriptional dynamics within the MRI-H186 cell line.
Organism	Human
Tissue	Cervix
Disease	Squamous cell carcinoma
Synonyms	Cerv-186, MRI-H-186, MRI-H186

Age	42 years
Gender	Female
Ethnicity	African
Morphology	Epithelial-like
Growth properties	Adherent

Citation	CERV-186 (Cytion catalog number 300290)
Biosafety level	2
NCBI_TaxID	9606
CellosaurusAccession	CVCL_5720

Tumorigenic	Yes, in nude mice
Viruses	HPV-16 positive
Products	Cytokeratine 8, 18, Vimentin, Desmoplakin

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	2 x 10⁴ cells/cm² will result in a confluent monolayer within 7 days
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300290-515SF	Certificate of Analysis	23. May. 2025	300290

CERV-186 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)