CEM/C1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305103

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The CEM/C1 cell line is a derivative of the CCRF-CEM human T-cell leukemia cell line, specifically selected for its resistance to certain chemotherapeutic agents, notably the topoisomerase II inhibitor, doxorubicin. This selection confers the cell line with significant applications in the study of multidrug resistance, a prevalent challenge in the treatment of various cancers. The CEM/C1 line exhibits overexpression of the MDR1 gene, which encodes the P-glycoprotein, a key efflux transporter involved in the resistance of cells to chemotherapeutic drugs. Genetically, CEM/C1 cells are characterized by their human T-lymphoblastoid lineage, making them highly relevant for research into T-cell biology and leukemia. The cells maintain a robust proliferative capacity and can be used in in vitro experiments aimed at understanding the cellular mechanisms of drug resistance, apoptosis, and the efficacy of new chemotherapeutic agents. These cells also provide a valuable tool for pharmacological studies, particularly in evaluating the pharmacodynamics and pharmacokinetics of anticancer drugs within a controlled experimental setting. Due to their drug-resistant properties, CEM/C1 cells are particularly useful in the development of treatment strategies that circumvent or directly target mechanisms of drug resistance. Studies utilizing this cell line can contribute to the broader understanding of cancer cell survival tactics and potentially lead to the development of more effective cancer therapies, especially for refractory or relapsed T-cell leukemia.
Organism	Human
Tissue	Peripheral blood
Disease	T-cell acute lymphoblastic leukemia
Synonyms	CCRF-CEM C1, CEM-C1, CEM.C1, CEMC1

Age	4 years
Gender	Female
Morphology	Lymphoblast
Growth properties	Suspension

Citation	CEM/C1 (Cytion catalog number 305103)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_3496

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS
Subculturing	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10⁵ cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305103-181223	Certificate of Analysis	23. May. 2025	305103

CEM/C1 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)