CCK-81 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$500.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305757

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The CCK-81 cell line is a human colorectal adenocarcinoma model derived from a primary tumor. It is commonly used in cancer biology studies focusing on gastrointestinal malignancies and has been characterized for various genetic alterations and drug response profiles. According to functional screening of tumor suppressor genes, CCK-81 expresses mutant TP53, as confirmed by yeast-based functional assays, with only about 6% of colonies showing transcriptionally active p53 phenotype, indicating a loss-of-function mutation. This mutation status aligns with its tumorigenic origin and contributes to its relevance as a model for studying p53-deficient colorectal cancers. CCK-81 has also been included in major cancer cell line compendia such as the Cancer Cell Line Encyclopedia (CCLE), where it has been profiled across multiple omics layers including gene expression, copy number variation, mutation status, and drug sensitivity. Its inclusion in these datasets allows for integrated analyses of pathway dependencies and therapeutic vulnerabilities across colorectal cancer subtypes. For instance, proteogenomic profiling has highlighted that colorectal cancer cell lines, including CCK-81, often exhibit dysregulated signaling pathways such as Wnt/β-catenin and MAPK, making them suitable for precision oncology studies targeting these axes.
Organism	Human
Tissue	Metastatic
Disease	Colon adenocarcinoma
Metastatic site	Lymph node
Synonyms	CCK81

Age	62 years
Gender	Female
Ethnicity	Japanese
Growth properties	Adherent

Citation	CCK-81 (Cytion catalog number 305757)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_2873

Mutational profile	Mutation: FBXW7, Simple, p.Arg465Cys (c.1393C>T), Heterozygous (DepMap=ACH-000963).Mutation, PIK3CA, Simple, p.Cys420Arg (c.1258T>C), Heterozygous (DepMap=ACH-000963).Mutation, TP53, Simple, p.Pro278His (c.833C>A), Heterozygous

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS, 1% NEAA, 1mM Sodiumpyruvat
Dissociation Reagent	Accutase
Doubling time	45 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 to 3 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Properties "CCK-81 Cells"

Metastatic site:	Lymph node

Lot Number	Certificate Type	Date	Catalog Number
305757-300426	Certificate of Analysis	24. Jun. 2026	305757

CCK-81 Cells

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Certificate of Analysis (CoA)