CADO-ES1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300127

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The CADO-ES1 cell line was established from a malignant pleural effusion taken from a 19-year-old female patient diagnosed with Ewing's sarcoma, primarily located in the right buttock with multiple lung metastases. This cell line provides a valuable tool for research in sarcoma biology, particularly in studying the metastatic processes associated with Ewing's sarcoma. As a disease primarily affecting children and young adults, Ewing's sarcoma is characterized by small round cells that are highly malignant, often exhibiting aggressive behavior and poor prognosis, particularly when metastatic. Uniquely, CADO-ES1 cells exhibit several critical features valuable for in-depth cancer research. They are heterotransplantable, meaning they can be transplanted into a different species (e.g., mice), which is vital for in vivo studies. This capacity makes them a robust model for studying tumor growth and metastasis in a controlled, yet biologically relevant system. Additionally, these cells have shown the ability to grow independently of anchorage, a characteristic typical of many cancerous cells which allows them to thrive without adhering to the extracellular matrix. Furthermore, CADO-ES1 cells can differentiate neurally in response to cyclic AMP (cAMP), providing a unique perspective into the cellular behaviors influenced by signaling pathways in cancer progression and differentiation. This combination of features makes CADO-ES1 a significant model for not only understanding the pathology of Ewing's sarcoma but also for the development and testing of targeted therapies that might inhibit the growth and spread of similar cancers. Research utilizing this cell line can contribute to a deeper understanding of cancer cell behavior, metastatic mechanisms, and potential therapeutic targets in sarcomas.
Organism	Human
Tissue	Bone
Disease	Ewing's Sarcoma
Synonyms	CADO-ES-1, CADO ES1, CADOES1, CADO-ES, Cado-ES, ESCADO1, Center for Adult Diseases Osaka-Ewing Sarcoma 1

Age	19 years
Gender	Female
Ethnicity	Japanese
Morphology	Small round cells
Growth properties	Monolayer, adherent

Citation	CADO-ES1 (Cytion catalog number 300127)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1103

Receptors expressed	CD99 (Eun Jung Lee, 2003)

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	Every 3 to 4 days
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300127-270126	Certificate of Analysis	20. Feb. 2026	300127
300127-290925	Certificate of Analysis	18. Dec. 2025	300127

CADO-ES1 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)