C643 Cells
General information
Description | The cell line C643 was established from a fine-needle biopsy of an anaplastic thyroid carcinoma of a 76-year-old man by Mark et al. in 1987. The patient died within 5 months after diagnosis. Demonstration of thyroglobulin mRNA ascertained a thyroid epithelial origin of the cell line. C643 cells emerge as a valuable tool for thyroid cancer research. These cells originated from human thyroid cancer tissue and represented metastatic PTC, FTC, and ATC. Their genetic makeup reflects the common mutations observed in thyroid cancer, such as alterations in BRAF, RAS, and PI3K genes, which activate critical signalling pathways. This makes C643 cells an ideal model for investigating the mechanisms involved in thyroid cancer development and progression. Furthermore, C643 cells are a crucial resource for testing potential targeted therapies. Their inclusion in preclinical studies can aid in identifying and evaluating novel compounds that specifically target the altered signalling pathways implicated in thyroid cancer. By accurately representing human thyroid cancer, C643 cells contribute to developing more effective treatments for patients with advanced thyroid cancer. |
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Organism | Human |
Tissue | Thyroid gland anaplastic |
Disease | Anaplastic thyroid carcinoma |
Synonyms | C 643, C-643, c643 |
Characteristics
Age | 76 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | C643 (Cytion catalog number 300298) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:5 to 1:10 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will yield in a confluent layer in about 3 days |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,11
D13S317: 8, 10
D16S539: 9, 13
D5S818: 11, 12
D7S820: 9, 12
TH01: 9.3, 10
TPOX: 11, 12
vWA: 15, 17
D3S1358: 15
D21S11: 28
D18S51: 14, 18
Penta E: 5, 15
Penta D: 9
D8S1179: 11, 13
FGA: 18, 21
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