C127I Cells
General information
Description | The murine cell line C127I, derived from malignant neoplasms of the mammary gland, exhibits a distinctive epithelial nature, rendering it a valuable asset for a variety of applications in research. Notably, C127I cells have undergone genetic modification through the introduction of a chimeric plasmid comprising bovine papillomavirus and the human interferon (HuIFN) gene, particularly IFN-gamma or IFN-alpha 5, regulated by the Simian virus 40 early promoters. This genetic transformation results in the maintenance of 30-50 extrachromosomal copies of the hybrid plasmid and the continuous secretion of elevated levels of HuIFNs. As a result, Mouse C127I Cells present an exciting opportunity for researchers to investigate the expression and functions of diverse proteins and their implications on biological systems, offering a valuable model system for studying mammary biology and related diseases. For example, C127I cells serve as a versatile platform for various applications, including acting as a transfection host for the transformation with bovine papillomavirus DNA plasmids, facilitating the visualization of sarcoma virus-induced foci, and enabling quantitative in vitro assays for bovine papilloma virus research, further enhancing their utility in scientific investigations. |
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Organism | Mouse |
Tissue | Breast, mammary gland |
Disease | Carcinoma |
Applications | Transfection host for transformation with bovine papilloma virus DNA plasmids. Visualization of sarcoma virus-induced foci. Quantitative in vitro assays for bovine papilloma virus. |
Synonyms | C 1271, C-127I, C-127 I, CNC 127I |
Characteristics
Gender | Female |
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Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | C127I (Cytion catalog number 400134) |
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Biosafety level | 1 |
Expression / Mutation
Viruses | Negative for ectromelia virus (mousepox). |
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Virus susceptibility | Bovine papilloma virus |
Reverse transcriptase | Negative (as determined in supernatant fluid) |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:5 to 1:15 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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