BJAB Cells
General information
Description | Established 1973 from a 5-year-old African girl with a (EBV-negative) Burkitt?s Lymphoma. The cell line was reported to be EBV-negative |
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Organism | Human |
Tissue | Blood |
Disease | Burkitt lymphoma |
Applications | Analysis of B cell surface antigens, testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, HLA-typing |
Synonyms | BJAb, BJA-B, BJAB-1, BJA-B1, BJA-B-1 |
Characteristics
Age | 5 years |
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Gender | Female |
Ethnicity | African |
Morphology | Round cells |
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | BJAB (Cytion catalog number 302006) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD10+, CD19+, CD20+, CD21(+), CD22+, CD23-, CD24-, CD32+, CD37+, CD38+, CD39-, CD40+, CD54+, CD72+, CD73-, CD75+, CD77+, CD81, CD82+, CD83+, CD84+, CD86+ |
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Karyotype | 46, hypodiploid |
Handling
Culture Medium | RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3 (Cytion article number 820702a) |
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Medium supplements | Supplement the medium with 20% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 3 x 10^5 cells/ml |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | Allow the cells to recover from the freezing process for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 8, 10
D13S317: 9,11
D16S539: 9, 12
D5S818: 12, 13
D7S820: 10, 11
TH01: 7
TPOX: 6, 9
vWA: 14, 15
D3S1358: 16
D21S11: 27, 28
D18S51: 16, 22
Penta E: 7
Penta D: 10, 11
D8S1179: 14, 18
FGA: 27, 28
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HLA alleles |
A*: 01:01:83, 02:01:01
B*: 13:02:01, 35:01:01
C*: 04:01:01, 06:02:01
DRB1*: 12:01:01, 13:02:01
DQA1*: 01:02:01, 05:05:01
DQB1*: 03:01, 06:04:01
DPB1*: 04:02:01G
E: 01:01, 01:03
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