BALL-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305084

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The BALL-1 cell line originates from a 75-year-old male patient diagnosed with acute lymphoblastic leukemia (ALL). Established from the peripheral blood, this cell line is of particular interest due to the patient's advanced age, offering a unique perspective on the disease in elderly populations. BALL-1 cells exhibit characteristics of B-cell lineage, notably expressing markers such as CD19 and CD10. These cells are negative for surface immunoglobulin, aligning with phenotypes observed in early stages of B-cell neoplastic development. As a model, BALL-1 is pivotal for researching the pathogenesis of B-cell leukemia, particularly within older patients, where disease dynamics may differ significantly from those observed in younger individuals. This cell line facilitates the exploration of molecular and cellular mechanisms underlying leukemia progression, therapeutic resistance, and the emergence of new drug targets. BALL-1 is instrumental in drug discovery and testing, aiding in the assessment of new anti-leukemic compounds. Moreover, the genetic abnormalities present in BALL-1 provide essential insights into the chromosomal alterations involved in the pathogenesis of B-cell precursor acute lymphoblastic leukemia.
Organism	Human
Tissue	B Lymphocyte
Disease	B-cell acute lymphoblastic leukemia
Synonyms	Ball-1, Ball 1, BALL1, B-cell Acute Lymphoblastic Leukemia-1

Age	75 years
Gender	Male
Ethnicity	Asian
Morphology	Lymphoblast
Growth properties	Suspension

Citation	BALL-1 (Cytion catalog number 305084)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1075

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS
Doubling time	48 to 72 hours
Subculturing	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10⁵ cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
Seeding density	An initial seeding density of 5 x 10⁵ cells/mL is recommended. A seeding density of 2 x 10⁵ cells/mL is recommended to maintain the culture.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305084-150324	Certificate of Analysis	23. May. 2025	305084

BALL-1 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)