BALL-1 Cells
General information
Description | This cell line was established in 1976 from the peripheral blood of a 75-year-old male diagnosed with acute lymphoblastic leukemia (ALL). |
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Organism | Human |
Tissue | B Lymphocyte |
Disease | B-cell acute lymphoblastic leukemia |
Synonyms | Ball-1, Ball 1, BALL1, B-cell Acute Lymphoblastic Leukemia-1 |
Characteristics
Age | 75 years |
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Gender | Male |
Ethnicity | Asian |
Morphology | Lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | BALL-1 (Cytion catalog number 305084) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Doubling time | 48 to 72 hours |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Split ratio | 1: 2 to 1: 4 |
Seeding density | An initial seeding density of 5 x 10^5 cells/mL is recommended. A seeding density of 2 x 10^5 cells/mL is recommended to maintain the culture. |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,12
D13S317: 9,12
D16S539: 9
D5S818: 10,13
D7S820: 10,12
TH01: 7,9
TPOX: 8,11
vWA: 14,18
D3S1358: 16
D21S11: 30
D18S51: 12,13
Penta E: 14,16
Penta D: 9,10
D8S1179: 10,14
FGA: 22,23
D6S1043: 12,18
D2S1338: 19,22
D12S391: 19,20
D19S433: 13,15.2
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