B-LCL-HROC313 (Bc HROC313) Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
---|---|
Tissue | Peripheral blood |
Disease | Carcinoma |
Synonyms | Bc HROC313 |
Characteristics
Morphology | Round cells |
---|---|
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | B-LCL-HROC313 (Cytion catalog number 302058) |
---|---|
Biosafety level | 2 |
Depositor | M. Linnebacher |
Expression / Mutation
Surface antigens | CD19 |
---|
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12,13
D5S818: 11,13
D7S820: 10
TH01: 6,9.3
TPOX: 8,11
vWA: 14,15
D3S1358: 14,15
D21S11: 29,31.2
D18S51: 12,16
Penta E: 12,13
Penta D: 11,16
D8S1179: 13
FGA: 20,24
|
HLA alleles |
A*: 01:01:01, 02:01:01
B*: 08:01:01, 40:01:02
C*: 03:04:01, 07:01:01
DRB1*: 03:01:01, 08:01:01
DQA1*: 04:01:01, 05:01:01
DQB1*: 02:01:01, 04:02:01
DPB1*: 04:01:01G, 04:02:01
E: 01:01:01
|