B-LCL-HROC103 Cells
General information
Organism | Human |
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Tissue | Peripheral blood |
Disease | Carcinoma |
Synonyms | Bc HROC103 |
Characteristics
Age | Age unspecified |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | B-LCL-HROC103 (Cytion catalog number 302020) |
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Biosafety level | 2 |
Depositor | M. Linnebacher |
Expression / Mutation
Surface antigens | CD19 |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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HLA alleles |
A*: 01:01:01, 02:01:01
B*: 07:02:01, 13:02:01
C*: 06:02:01, 07:02:01
DRB1*: 07:01:01, 15:01:01
DQA1*: 01:02:01, 02:01:01
DQB1*: 02:02:01, 06:02:01
DPB1*: 04:01:01, 13:01:01G
E: 01:01:01
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