B-LCL-HROC102 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
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Tissue | Peripheral blood |
Disease | Carcinoma |
Synonyms | Bc HROC102 |
Characteristics
Age | Age unspecified |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | B-LCL-HROC102 (Cytion catalog number 302001) |
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Biosafety level | 2 |
Depositor | M. Linnebacher |
Expression / Mutation
Surface antigens | CD19 |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 10,13
TH01: 6,9.3
TPOX: 8
vWA: 14,17
D3S1358: 15,17
D21S11: 30,33.2
D18S51: 14
Penta E: 12,13
Penta D: 8,12
D8S1179: 13,15
FGA: 21,25
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