B-LCL-HROC06 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
---|---|
Tissue | Peripheral blood |
Disease | Carcinoma |
Synonyms | Bc HROC06 |
Characteristics
Age | Age unspecified |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | B-LCL-HROC06 (Cytion catalog number 302065) |
---|---|
Biosafety level | 2 |
Depositor | M. Linnebacher |
Expression / Mutation
Surface antigens | CD19 |
---|
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 12, 12
D13S317: 11, 13
D16S539: 11, 12
D5S818: 9, 13
D7S820: 10, 12
TH01: 6, 9.3
TPOX: 8, 8
vWA: 16, 16
D3S1358: 15, 15
D21S11: 27, 30
D18S51: 18, 18
Penta E: 12, 12
Penta D: 10, 12
D8S1179: 14, 14
FGA: 22, 23
|