AT-1 Cells
General information
Description | The AT-1 cell line was derived from a spontaneous Dunning R-3327 rat prostatic cancer. The cell line is anaplastic and has low metastatic ability. |
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Organism | Rat |
Tissue | Prostate |
Disease | Adenocarcinoma |
Synonyms | R-3327-AT-1, AT1, AT-1-TC, Dunning R-3327 AT-1, R3327-AT1 |
Characteristics
Morphology | Epithelial-like |
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Growth properties | Adherent. The cells form clusters in soft agar and can be adapted to suspension growth |
Identifiers / Biosafety / Citation
Citation | AT-1 (Cytion catalog number 500121) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in rat and nude mice |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 4 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D1Wox31: 100
Rat_D2Wox37: 156
Rat_D19Wox11: 228
Rat_D10Wox8: 266
Rat_D4Wox7: 145
Rat_D2Wox27: 223
Rat_D5Rat33: 134,136
Rat_D10Wox11: 171
Rat_D1Wox23: 226
Rat_D12Wox1: 410
Rat_D6Wox2: 112
Rat_D8Wox7: 179
Rat_D6Cebr1: 223
SRY: x,x
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