AR42J Cells
General information
Description | AR42J cells are a rat pancreatic tumor cell line derived from azaserine-induced tumors in rats. They are widely used as a model for studying pancreatic exocrine cell functions, pancreatitis, and pancreatic cancer research. AR42J cells exhibit acinar-like characteristics, making them particularly valuable for investigating the physiology and pathology of pancreatic acinar cells. One of the defining features of AR42J cells is their ability to differentiate into cell types exhibiting more pronounced pancreatic exocrine functions when treated with various agents, such as dexamethasone or activators of protein kinase C. Upon differentiation, these cells produce and secrete digestive enzymes, including amylase, lipase, and chymotrypsin, mimicking the enzyme secretion profile of normal pancreatic acinar cells. AR42J cells are also used to explore the mechanisms of acute pancreatitis. They respond to stimuli like cerulein, a cholecystokinin analog, which can induce a condition in the cells that resembles acute pancreatitis, characterized by enzyme overproduction, oxidative stress, and inflammatory responses. This makes AR42J cells a useful tool for testing potential therapeutic interventions for pancreatitis. Furthermore, the AR42J cell line is utilized in research focused on pancreatic cancer, particularly for studies on tumorigenesis and the malignant transformation of acinar cells. They are instrumental in examining the effects of oncogenes, tumor suppressor genes, and growth factors on the development and progression of pancreatic cancer. Overall, AR42J cells provide a versatile and dynamic model system for advancing our understanding of pancreatic diseases and for the development of new therapeutic strategies targeting these conditions. |
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Organism | Rat |
Tissue | Pancreas tumor, exocrine |
Disease | Neoplasia |
Synonyms | AR4-2J, AR-42J |
Characteristics
Morphology | Epithelial-like |
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Growth properties | Cells grow slowly, in clusters and appear as hollow spheroid colonies. They can pile up and attach loosely. |
Identifiers / Biosafety / Citation
Citation | AR42J (Cytion catalog number 500478) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Insulin, glucocorticoid |
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Tumorigenic | Yes, in athymic mice |
Products | Amylase and other exocrine enzymes |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | It is recommended to cover the tissue culture flasks with gelatine prior to cell cultivation. Gelatine is added to the flask, incubated for 30min at 37 degree Celsius and washed once with PBS. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D1Wox31: 104
Rat_D2Wox37: 156
Rat_D19Wox11: 232
Rat_D10Wox8: 266
Rat_D4Wox7: 153,157
Rat_D2Wox27: 207
Rat_D5Rat33: 122,124
Rat_D10Wox11: 171
Rat_D1Wox23: 210,214
Rat_D12Wox1: 402,406
Rat_D6Wox2: 104
Rat_D8Wox7: 182
Rat_D6Cebr1: 233,241,243
SRY: x,Y
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