A704 Cells
General information
Description | A-704 is a human epithelial cell line derived from kidney tissue of a 78-year-old male patient with adenocarcinoma. This cell line exhibits an epithelial morphology. It is a valuable resource in cancer research, particularly for studying adenocarcinoma. A-704 is a versatile cell line with applications in 3D cell culture and as a transfection host. Derived by D.J. Giard, A-704 maintains consistency and reliability in experimental settings. Karyotype analysis reveals that A-704 cells exhibit abnormalities such as breaks, dicentrics, and endoreduplication, ranging from diploid to hyperdiploid, hypertriploid to hypertetraploid. While not tumorigenic in immunosuppressed mice, A-704 cells can form colonies in a semisolid medium. A-704 cells exhibit specific isoenzyme profiles, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3. |
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Organism | Human |
Tissue | Kidney |
Disease | Adenocarcinoma |
Synonyms | A.704, A-704 |
Characteristics
Age | 78 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | A704 (Cytion catalog number 300217) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | Me-2, 1, PGM3, 1-2, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B |
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Tumorigenic | No |
Karyotype | (P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will result in a confluent monolayer within 4 days. |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 7,8
D13S317: 8
D16S539: 12,13
D5S818: 10,11
D7S820: 10
TH01: 7,9
TPOX: 11
vWA: 14,18
D3S1358: 15
D21S11: 28,32
D18S51: 16,17
Penta E: 8,17
Penta D: 2.2,11
D8S1179: 13,15
FGA: 22,23
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HLA alleles |
A*: 34:02:01, 74:01:01
B*: 35:01:01, 44:03:01
C*: 04:01:01
DRB1*: 15:03:01G
DQA1*: 01:02:01
DQB1*: 06:02:01
DPB1*: 02:01:19, 04:02:01G
E: 01:01:01, 01:03
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