A673 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300454

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The A673 cell line is a valuable resource in biological science. Derived from the muscle tissue of a 15-year-old female patient diagnosed with Ewings Sarcoma, this cell line exhibits a distinct polygonal morphology. Originally the cell line was thought to be derived from a rhabdomyosarcoma (RMS). One of the remarkable characteristics of A673 cells is their ability to produce several growth factors that possess oncogenic potential. These cells also secrete growth-inhibitory factors, providing a balanced environment for cellular growth regulation. Such properties make A673 cells an excellent model for investigating the interplay between tumour-promoting and tumour-suppressing factors. A673 cells have demonstrated tumorigenic potential, as they can induce tumour formation in immunosuppressed mice. Moreover, studies have identified hypermethylated promoters in cancer-related genes within the A673 cell line. These genetic alterations further contribute to its relevance in cancer research, offering a platform to explore epigenetic modifications and their impact on tumour development and progression. While A673 cells are often referred to as Ewing tumour (ET) or sarcoma (ES), they are also associated with rhabdomyosarcoma (RMS). Notably, the A673 cell line harbours a complex karyotype with a specific translocation involving chromosomes 11 and 22. This translocation leads to the fusion of the EWS and FLI1 genes, which is a characteristic genetic event in Ewing Tumor.
Organism	Human
Tissue	Bone
Disease	Ewing's Sarcoma
Synonyms	A-673, RMS 1598, RMS1598

Age	15 years
Gender	Female
Ethnicity	Caucasian
Morphology	Fibroblast-like
Growth properties	Monolayer, adherent

Citation	A673 (Cytion catalog number 300454)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0080

Tumorigenic	Yes, in immunosuppressed mice
Virus susceptibility	Highly sensitive to human adenoviruses

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	28 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm² will result in a confluent monolayer within 8 days.
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300454-3d21	Certificate of Analysis	26. May. 2025	300454

A673 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)