A549-Luc Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305648

Safety data sheet

Product sheet

Description	A549-Luc is a genetically engineered derivative of the human lung adenocarcinoma cell line A549, modified to stably express the firefly luciferase reporter gene. The parental A549 cell line originates from a lung carcinoma of an adult patient and is widely used as a model for non-small cell lung cancer (NSCLC), particularly adenocarcinoma. A549 cells exhibit epithelial morphology and harbor key molecular features relevant to lung cancer biology, including mutations in KRAS and alterations in pathways regulating proliferation, metabolism, and response to oxidative stress. The luciferase-expressing variant retains these intrinsic characteristics while enabling bioluminescent detection. The incorporation of the luciferase gene allows for sensitive, non-invasive quantification of viable cells through bioluminescence imaging following administration of a luciferin substrate. This enables real-time monitoring of cell proliferation, tumor growth, and therapeutic response in both in vitro assays and in vivo xenograft models. The emitted light signal correlates with metabolically active cell number, making A549-Luc particularly suitable for longitudinal studies of tumor kinetics, metastasis, and drug efficacy. Stable integration ensures sustained reporter expression, although signal intensity may vary depending on clone selection and experimental conditions. A549-Luc maintains the biological behavior of the parental A549 line, including its utility in studies of lung cancer progression, inflammation, and response to chemotherapeutic or targeted agents. The addition of a luciferase reporter significantly enhances experimental flexibility, supporting high-throughput drug screening, in vivo imaging, and quantitative assessment of tumor burden over time. As such, A549-Luc is a valuable tool for translational lung cancer research and preclinical evaluation of novel therapeutic strategies.
Organism	Human
Tissue	Lung
Disease	Lung adenocarcinoma

Age	58 years
Gender	Male
Ethnicity	Caucasian
Growth properties	Adherent

Citation	A549-Luc (Cytion catalog number 305648)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_J242
GMO Status	GMO-S1: This human A549 lung carcinoma line contains a lentiviral-Luc reporter construct enabling bioluminescent monitoring of tumor response. This classification applies only within Germany and may differ elsewhere.

Mutational profile	Mutation: p.Gly12Ser, Homozygous; Mutation: p.Gln37Ter, Homozygous

Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

A549-Luc Cells

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Regulatory Data

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