A204 Cells
General information
Description | A204 cells are human epithelial cells derived from the muscles of a 1-year-old female patient with rhabdomyosarcoma. With applications in 3D cell culture and tumorigenic properties, A-204 cells provide an opportunity for studying tumour biology and potential therapeutic interventions. Derived from muscle tissue, A-204 cells closely resemble the outer layer of cells found in organs and tissues. A-204 cells exhibit adherent growth, have an epithelial morphology and have a karyotype characterized by diploidy and tetraploidy. Furthermore, these cells have demonstrated tumorigenicity, forming malignant tumours consistent with embryonal rhabdomyosarcoma in mice, validating their relevance in cancer research. The presence of specific isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3, in A-204 cells provides insight into their metabolic characteristics. These isoenzymes may play a role in understanding cellular processes involved in cancer progression and treatment response. With a doubling time of approximately 36 hours, A-204 cells exhibit rapid proliferation, allowing efficient experimentation and ensuring an ample supply of cells for research applications. |
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Organism | Human |
Tissue | Muscle |
Disease | Rhabdomyosarcoma |
Synonyms | A-204 |
Characteristics
Age | 1 year |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | A204 (Cytion catalog number 300109) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | PGM3, 1, PGM1, 1, ES-D, 1, Me-2, 1, AK-1, 1, GLO-1, 1, G6PD, B |
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Tumorigenic | In nude mice. Forms small malignant tumors which are conform to embryonal rhabdomyosarcoma. |
Ploidy status | Diploid and tetraploid |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 26 to 36 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:6 to 1:10 is recommended |
Seeding density | 0.5 to 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 2 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,13
D13S317: 11,12
D16S539: 11,12
D5S818: 12
D7S820: 8,1
TH01: 8,9.3
TPOX: 8,9
vWA: 15,17
D3S1358: 14,17
D21S11: 28,3
D18S51: 17,18
Penta E: 7,1
Penta D: 9,12
D8S1179: 13,15
FGA: 21
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