3T3-Swiss albino Cells
General information
Description | The 3T3-Swiss Albino cell line is a fibroblast cell line derived from the tissues of a Swiss albino mouse embryo. Developed in the 1960s by George Todaro and Howard Green, this line was one of the first to be established for the purpose of long-term cultivation and research of fibroblast cells. The name "3T3" refers to the protocol used for subculturing these cells: "3" days interval and "T3" for the population density at which cells were seeded (3 x 10^5 cells per 20 cm? flask). 3T3-Swiss Albino cells are commonly used as a model system for studying fibroblast biology, including cellular aging, transformation, and the effects of various pharmaceuticals and toxins on cellular health and replication. They are particularly noted for their robustness and reliability in supporting the replication of various mammalian viruses and for producing viral vaccines. Additionally, these cells are instrumental in cancer research, providing a consistent model for examining the cellular mechanisms of oncogenesis and the interaction of cancer cells with connective tissue environments. Genetically, 3T3-Swiss Albino cells are characterized by a stable karyotype, which facilitates their use in genetic studies. They are highly adaptable to various in vitro conditions, making them extremely valuable for genetic, cytological, and biochemical studies. Their role in the development of biomedical research cannot be overstated, providing crucial insights into cellular processes and potential therapeutic targets in various diseases. |
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Organism | Mouse |
Tissue | Embryonic |
Applications | This cells have been used to study cancer development and progression, embryonic development and differentiation, signaling pathways involved in cellular processes such as cell growth and differentiation, and as a substrate for the production of monoclonal antibodies and the expression of recombinant proteins for production and purification. |
Synonyms | 3T3 Swiss Albino, 3T3, Swiss-3T3, Swiss 3T3, Swiss3T3 |
Characteristics
Age | Embryo |
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Gender | Male |
Morphology | Fibroblast-like |
Cell type | Fibroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | 3T3-Swiss Albino (Cytion catalog number 400103) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | No |
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Viruses | Tested and found negative for ectromelia virus (mousepox). |
Virus susceptibility | Polyomavirus, SV40 |
Reverse transcriptase | Negative |
Products | t |
Ploidy status | Hypertriploid |
Karyotype | 2n=40 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 18 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:5 to 1:10 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will result in a confluent monolayer within 4 days. |
Fluid renewal | 2 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 19
M_4-2: 19.3
M_6-7: 12
M_3-2: 15
M_19-2: 11,12
M_7-1: 29
M_1-1: 10,11
M_8-1: 15
M_2-1: 9
M_15-3: 20.3
M_6-4: 15.3
M_11-2: 15,16,17
M_1-2: 13,17,18
M_17-2: 14
M_12-1: 20,21
M_5-5: 14,15
M_X-1: 25
M_13-1: 16.2
Human D4/D8: -
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