SW1271 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$1,104.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305880

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The SW1271 cell line is a human small cell lung carcinoma (SCLC) model derived from an adult patient. It is characterized by its neuroendocrine phenotype, which is typical of SCLC, and displays molecular features relevant to therapeutic sensitivity and resistance. In a comprehensive epigenome-wide methylation analysis of SCLC cell lines, including SW1271, the line exhibited specific DNA methylation patterns that correlated with chemosensitivity to several classes of anticancer drugs. These included Aurora kinase inhibitors, CDK inhibitors, and DNA-damaging agents. The methylation status of key genes such as TREX1, SLFN11, CEP350, and KDM1A in SW1271 and other SCLC models has been associated with altered drug response, implicating epigenetic modulation as a determinant of therapeutic efficacy. Furthermore, SW1271 has been used in integrated genomic and epigenomic studies to understand subtype-specific vulnerabilities in SCLC. This cell line, along with others representing different SCLC transcriptional subtypes (ASCL1, NEUROD1, POU2F3, and YAP1), helps delineate the heterogeneity within the disease. The methylation profile of SW1271 contributes to our understanding of the regulatory mechanisms affecting gene expression and drug response, including suppression of tumor suppressor genes and dysregulation of lineage-specific transcription factors. These insights position SW1271 as a valuable model for investigating epigenetically driven pathways in SCLC and for identifying potential biomarkers and therapeutic targets.
Organism	Human
Tissue	Lung
Disease	Lung small cell carcinoma
Synonyms	SW-1271, SW 1271

Age	69 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial
Cell type	Epithelial cell
Growth properties	Adherent

Citation	SW1271 (Cytion catalog number 305880)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1716

Antigen expression	Blood Type A; Rh +
Mutational profile	Mutation: NRAS, Simple, p.Gln61Arg (c.182A>G), Homozygous, SMARCA4, Simple, p.Asn774Lys (c.2322C>A), Homozygous.Mutation, TP53, Simple, p.Cys277Phe (c.830G>T), Homozygous

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 10% FBS, AB, 5µg/mL Insulin
Dissociation Reagent	Accutase
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305880-191125	Certificate of Analysis	08. Jan. 2026	305880

SW1271 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Organism	Human
Tissue	Colon
Disease	Adenocarcinoma, Grade IV, Dukes' type B.