SNU-387 Cells

CAD$759.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Product number: 305124

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The SNU-387 cell line is derived from a human hepatocellular carcinoma (HCC) and is widely utilized in liver cancer research. This cell line provides a valuable model for studying the molecular and cellular mechanisms of hepatocarcinogenesis, tumor progression, and therapeutic responses. Hepatocellular carcinoma is one of the most common and lethal forms of liver cancer, making cell lines like SNU-387 essential for advancing our understanding of the disease and developing effective treatments. SNU-387 cells exhibit an epithelial morphology and express markers typical of liver cancer, such as alpha-fetoprotein (AFP) and hepatocyte-specific antigens. They are characterized by genetic and epigenetic alterations common in HCC, including mutations in key oncogenes and tumor suppressor genes. Researchers use SNU-387 cells to investigate signaling pathways involved in liver cancer, such as the Wnt/β-catenin, PI3K/Akt, and MAPK pathways. These cells are also employed in high-throughput drug screening assays and preclinical testing of chemotherapeutic agents and targeted therapies. Additionally, SNU-387 cells are used to study the mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the SNU-387 cell line in hepatocellular carcinoma research highlights its importance in advancing our knowledge of liver cancer biology and in the development of new therapeutic approaches for HCC patients.
Organism	Human
Tissue	Liver
Disease	Adult hepatocellular carcinoma
Synonyms	SNU387, NCI-SNU-387

Age	41 years
Gender	Female
Ethnicity	Asian
Morphology	Epithelial
Growth properties	Adherent

Citation	SNU-387 (Cytion catalog number 305124)
Biosafety level	2
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0250

Antigen expression	Blood Type O, Rh +
Viruses	HBV

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	61 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305124-100924	Certificate of Analysis	26. May. 2025	305124

SNU-387 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Organism	Human
Tissue	Stomach
Disease	Gastric signet ring cell adenocarcinoma