Calu-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300141

安全資料表

產品說明書

分析證明書 (CoA)

說明	The Calu-1 cell line originates from human lung carcinoma, specifically non-small cell lung cancer (NSCLC). It was established from the pleural effusion of a 47-year-old Caucasian male with epidermoid carcinoma of the lung. This cell line exhibits epithelial-like morphology and has been used extensively in research focused on lung cancer biology, drug screening, and cytotoxicity studies. Calu-1 cells express several markers characteristic of lung epithelial cells and have been a valuable model for studying the molecular pathways involved in lung carcinogenesis and therapy resistance. Calu-1 cells are known for their high proliferation rate and robustness in culture, making them suitable for in vitro experimental setups. They retain several chromosomal abnormalities typical of cancer cells, which includes multiple copies of chromosomes 7 and 20, demonstrating their utility in genetic and cytogenetic studies. The cell line also exhibits mutations in key oncogenes and tumor suppressor genes like KRAS and TP53, respectively, which are of particular interest in lung cancer research. These genetic characteristics make Calu-1 a useful tool for investigating the impact of genetic alterations on cancer progression and for testing the efficacy of targeted therapies in a controlled environment.
生物體	Human
組織	Lung
疾病	Carcinoma
轉移部位	Pleural effusion
同義詞	CaLu-1, CALU-1, Calu.1, CALU 1, Calu 1, CALU1, Calu1

年齡	47 years
性別	Male
形態學	Epithelial-like
細胞類型	Epidermoid
生長特性	Adherent

引用	Calu-1 (Cytion catalog number 300141)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0608

蛋白質表達	P53 negative
抗原表達	Blood Type A, Rh+, HLA A10, A11, B15, Bw35
同工酶	Me-2, 1-2, PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0359
致癌基因	K-ras oncogene positive.
核型分析	The stem line chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells, M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. Y chromosome was not detected by QM band examination, although the cell line was initiated from a male.

培養基	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
營養補充品	Supplement the medium with 10% FBS and 1% NEAA
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm² will result in a 90% confluent monolayer in about 4 days
流體更新	2 to 3 times per week
解凍後的恢復	After thawing, plate the cells at 2 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300141-051023	分析證明書	23. May. 2025	300141
300141-080825	分析證明書	22. Oct. 2025	300141

Calu-1 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)