A375 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300110

安全資料表

產品說明書

分析證明書 (CoA)

說明	The A375 human melanoma cell line, isolated from the skin of a 54-year-old female patient with malignant melanoma, is a significant resource in cancer research, particularly in the study of human melanoma, one of the most aggressive forms of skin cancer. The A375 cell line is known for its rapid growth rate and high tumorigenic potential, making them suitable for various experimental applications, including in vitro studies on cell proliferation, migration, and invasion, as well as in vivo tumorigenesis assays. A375 cells exhibit high tumorigenic potential in immunosuppressed mice, forming rapidly growing amelanotic melanomas. The presence of the BRAFV600E mutation in A375 cells makes them highly sensitive to MEK inhibition, providing a valuable tool for investigating targeted therapies in melanoma treatment. The treatment of A375 cells with vemurafenib, for example, has been shown to enhance the induction of MHC Class I and Class II molecules, offering insights into the interactions between melanoma cells and the immune system. In addition to their role in basic melanoma research, A375 cells are used in drug screening and in the investigation of signaling pathways involved in cancer cell survival, proliferation, and metastasis. A375 cells have further been utilized in apoptosis studies and A375 isogenic cell lines and the introduction of reporter proteins like-Luc (luc2) enable the study of gene function and the monitoring of cellular responses in real-time. A375 cells' suitability as a transfection host and their use in stable reporter cell lines also contribute to their versatility in research applications. In summary, the A375 human melanoma cell line is a pivotal tool in the investigation of human melanoma, offering a comprehensive model for studying the molecular and cellular mechanisms underlying melanoma progression, the efficacy of therapeutic agents, and the interaction between cancer cells and the immune system.
生物體	Human
組織	Skin
疾病	Melanoma
同義詞	A 375, A-375, A375-MEL, A375-mel, A375mel

年齡	54 years
性別	Female
形態學	Epithelial-like
生長特性	Adherent

引用	A375 (Cytion catalog number 300110)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0132

抗原表達	P53 positive
致瘤性	Yes, in nude mice
突變譜	BRAF V600Emut
核型分析	A375 cells are characterized by their hypotriploid karyotype, with a modal chromosome number of 62, and the presence of nine marker chromosomes in each cell, highlighting the genetic alterations associated with malignant melanoma.

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
倍增時間	20 hours
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm² will result in a confluent monolayer within 4 days.
流體更新	2 to 3 times per week
解凍後的恢復	After thawing, plate the cells at 4 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300110-190525	分析證明書	21. Jul. 2025	300110
300110-931122w	分析證明書	15. Apr. 2025	300110

A375 Cells

Key facts about A375 cells

Properties of the melanoma cell line A375

Specifications

Genotype of the A375 cell line

Handling

Genetic profile

分析證明書 (CoA)