MCF10A Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305026

安全資料表

產品說明書

分析證明書 (CoA)

說明	The MCF10A human mammary epithelial cell line, established from the mammary gland of a 36-year-old female with fibrocystic disease, serves as a model for studying the intricacies of normal breast cell function, transformation, and the epithelial to mesenchymal transition critical in invasive breast carcinoma transition. As a non-tumorigenic epithelial cell line derived from benign proliferative breast tissue, MCF10A cells are instrumental in mammary cell studies, offering insights into breast tumor progression and the dynamics of tumor cells in mammospheres. MCF10 A cells, characterized by their three-dimensional growth in collagen and their ability to form acinar structures in mixed Matrigel, provide a reliable model for analyzing the impact of oncogenes and studying the mammosphere formation, which is crucial for understanding the properties of mammary progenitor cells and their role in cancer research. The MCF10A cell line, while exhibiting a basal-like phenotype, express a combination of luminal and stem-like markers, as well as epithelial-cell markers such as cytokeratins and milk proteins. Their responsiveness to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF) underscores the importance of growth factors and hormones in the proliferation and survival of human breast tissue cells. The MCF 10A model, provides a window into the genomic signaling pathways that govern cell behavior and phenotype in 3D culture, offering a platform for immunohistochemistry and immunofluorescence staining to visualize cellular processes. These cells are crucial for studying the transition of mammary cells during breast cancer development, including the role of lipid oxidation product genotoxicity and the impact of dietary components like soybean trypsin inhibitor on cell function. Furthermore, the MCF 10A cell line's comparison with other lines such as MCF7 (which is tumorigenic and estrogen receptor-positive) and MCF10F (another non-tumorigenic line but with different characteristics) enriches breast cancer research by providing diverse models for understanding the spectrum of non-invasive to highly metastatic phenotypes.
生物體	Human
組織	Mammary gland, breast
同義詞	MCF-10A, MCF 10A, MCF.10A, MCF10A, MCF10-A, MCF10a, MCF-10 Attached, Michigan Cancer Foundation-10A

年齡	36 years
性別	Female
形態學	Epithelial
生長特性	Adherent

引用	MCF10A (Cytion catalog number 305026)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0598

致瘤性	No

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 5% horse serum, 20 ng/mL EGF, 0,5 microgram/mL Hydrocortison, 10 microgram/mL Insulin. Add 100 ng/mL cholera toxin if needed.
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305026-131224	分析證明書	23. May. 2025	305026
305026-250123	分析證明書	23. May. 2025	305026
305026-250123SF	分析證明書	23. May. 2025	305026
305026-130426	分析證明書	15. May. 2026	305026

MCF10A Cells

Overview of the MCF 10A cell line

Aspects of MCF 10A epithelial cells

Documentation

Genomics of the human breast cancer cell line MCF 10A

Handling

Genetic profile of MCF10A cells

分析證明書 (CoA)

生物體	Human
組織	Breast
疾病	Adenocarcinoma

生物體	Human
組織	Breast
疾病	Adenocarcinoma