L929 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 400260

安全資料表

產品說明書

分析證明書 (CoA)

說明	L-929 cells are a fibroblast-like cell line derived from the subcutaneous connective tissue of a 100-day-old male C3H/An mouse. Established in the 1940s, this cell line has become pivotal in various biological and medical research fields due to its robustness, ease of culture, and versatility in applications. L-929 cells are characterized by their spindle-shaped, fibroblastic morphology, and adherent growth. They are widely used in cytotoxicity assays and serve as a standard model to assess the biocompatibility of materials and the toxic effects of various substances, which is particularly relevant in the fields of biomaterials and tissue engineering. L-929 cells are also employed in the study of cytokine activity, especially in assays for necrosis factor (TNF) activity, due to their sensitivity to TNF-induced cytotoxicity. This makes them valuable in immunology and inflammation research. L-929 cells are further utilized in virology as a host for viral replication studies. Their susceptibility to various viruses, such as the infectious bursal disease virus (IBDV), facilitates the investigation of viral life cycles, host-virus interactions, and the efficacy of antiviral compounds. Overall, the L-929 cell line is a valuable resource in scientific research and offers a versatile platform for studies in cytotoxicity, immunology, virology, and biomaterials.
生物體	Mouse
組織	Connective tissue, normal, subcutaneous, areolar and adipose
同義詞	NCTC clone 929, NCTC 929, NCTC-929, NCTC929, L cell, L cells, L-cell, L-cells, L cell line, L, Strain L-929, L 929, L929, L929(NCTC), Clone 929, Earles's cells, Earle's L cells

品種／亞種	C3H/An
年齡	100 days
性別	Male
形態學	Fibroblast-like
細胞類型	Fibroblast
生長特性	Adherent

引用	L-929 (Cytion catalog number 400260)
生物安全等級	1
NCBI_TaxID	10090
Cellosaurus 編號	CVCL_0462

抗原表達	H-2k
致瘤性	Yes, in immunosuppressed mice
病毒	Ectromelia virus (mousepox): negative
抗病毒性	Poliovirus 1, 2, 3, coxsackievirus B5, polyomavirus
逆轉錄酶	Positive

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
倍增時間	25 hours
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	2 to 3 x 10⁴ cells/cm²
流體更新	2 to 3 times per week
解凍後的恢復	24 to 48 hours
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
400260-921	分析證明書	23. May. 2025	400260
400260-161024	分析證明書	23. May. 2025	400260
400260-161024	分析證明書	05. Dec. 2025	400260
400260-150925	分析證明書	05. Dec. 2025	400260

L929 Cells

Key facts about L929 cells

Features of the mouse cell line L929

Specifications

Genotype

L929 cell purity and Identity checks

Quality control

分析證明書 (CoA)