HEK293-F Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300260

安全資料表

產品說明書

分析證明書 (CoA)

說明	HEK293-F cells are a fast-growing, highly transfectable subline derived from the human embryonic kidney 293 (HEK293) cell line. The 'F' designation indicates that these cells have been adapted for growth in suspension cultures, making them particularly useful for large-scale protein production. The cells grow in a variety of serum-free media, facilitating scalable processes in biotechnological and pharmaceutical applications. HEK293-F cells retain the epithelial-like morphology of the parent HEK293 line and are maintained in suspension without the need for attachment to a solid substrate. These cells are highly efficient at expressing recombinant proteins and are widely utilized in the production of viral vectors for gene therapy, including adenoviral, lentiviral, and retroviral vectors. Their robust growth in suspension and ease of transfection make them ideal for use in transient transfection protocols, where they can produce high yields of protein within a few days post-transfection. This characteristic is critical for rapid production cycles in research and industrial settings. The adaptability of HEK293-F cells to various growth conditions and their capacity for high-density culture enhance their utility in bioprocessing environments.
生物體	Human
組織	Kidney
應用	Transfection host
同義詞	HEK-293-F, HEK 293-F, HEK-293F, HEK293F, 293-F, 293 F, 293F

年齡	Fetus
性別	Female
形態學	Epithelial-like
生長特性	Suspension

引用	HEK293-F (Cytion catalog number 300260)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_6642
轉基因狀態	GMO-S1: This HEK293-F cell line contains SV40, enabling high transfection efficiency and robust growth in suspension culture. The modification is stably present in embryonic kidney cells. This classification applies only within Germany and may differ elsewhere.

受體的表達	Vitronectin
蛋白質表達	CEA negative, p53 positive
致瘤性	In nude mice
病毒	Transformed with adenovirus 5 DNA adenovirus 5 DNA

培養基	CD293 (Thermo)
解離試劑	Accutase
倍增時間	30 hours
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm² will yield in a confluent layer in about 4 days
流體更新	2 times per week
解凍後的恢復	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300260-170225SF	分析證明書	23. May. 2025	300260
300260-240326	分析證明書	04. May. 2026	300260

HEK293-F Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)