NIH-3T3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 400101

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	NIH-3T3 cells are a fibroblast cell line derived from the tissue of a NIH Swiss mouse embryo. These cells are known for their spindle-shaped morphology and are widely utilized in scientific research due to their ability to grow rapidly and to a high cell density. NIH-3T3 cells are particularly noted for their utility in genetic studies, including DNA transfection experiments, where they are used to introduce foreign DNA into their genomes. This has made them a valuable tool for studying gene function and regulation. Additionally, NIH-3T3 cells are employed in oncogenic research, specifically in assays for the identification and characterization of cancer-causing genes. They have a remarkable capacity to support the propagation of various types of viruses, including sarcoma and leukemia viruses, making them integral to virology studies. One of the key features of the NIH-3T3 cell line is its spontaneous immortalization. This characteristic, combined with their genetic stability over continuous passaging, makes NIH-3T3 cells an exemplary model system for exploring cellular processes, signaling pathways, and the effects of various pharmacological treatments in mammalian cells. Characterized by a heterogeneous cell population, NIH 3T3 mouse cells underscore the intrinsic cellular heterogeneity within fibroblast subtypes, which is critical for deciphering the complex interplay between cellular composition and tissue architecture. These cells exhibit a spindle-like morphology on a chitosan surface, transitioning to an elongated form on OCMCS (oxidized cellulose) surfaces. The NIH3T3 cell line ontology encompasses various subclones, including 3T3-L1, a model for adipogenesis, and 3T3-J2, employed as a feeder layer in keratinocyte cultures, illustrating the cellular line's broad applicability across different proliferation rates and research disciplines. NIH-3T3 cells are pivotal in research for their rapid growth, spindle-shaped morphology, and versatility in genetic and oncogenic studies. Their spontaneous immortalization and genetic stability enhance their utility in exploring cellular dynamics and pharmacological effects. The diversity within this cell line, including its response to various substrates and the existence of specialized subclones like 3T3-L1 and 3T3-J2, underscores its broad applicability and critical role in advancing our understanding of cellular behavior and disease mechanisms.
Organismo	Mouse
Tecido	Embryonic
Aplicações	Transfection host
Sinônimos	NIH/3T3, NIH 3T3, NIH3T3, 3T3, 3T3NIH, 3T3-Swiss, Swiss-3T3, Swiss/3T3, Swiss 3T3, Swiss3T3

Raça/Subespécie	NIH Swiss
Idade	Embryo
Gênero	Male
Morfologia	Spindle-like morphology, indicative of their fibroblast nature
Tipo de célula	Fibroblast
Propriedades de crescimento	Adherent

Referência	NIH-3T3 (Cytion catalog number 400101)
Nível de biossegurança	1
NCBI_TaxID	10090
Número de acesso do Cellosaurus	CVCL_0594

Vírus	MAP-test: Negative.

Meio de cultura	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
400101-1420	Certificado de Análise	23. May. 2025	400101
400101-161224	Certificado de Análise	18. Aug. 2025	400101
400101-190226	Certificado de Análise	25. Mar. 2026	400101

NIH-3T3 Cells

Essential facts about the NIH-3T3 cell line

Details

Specifications

Genetic profile of the fibroblast cell line

Culturing methods

Quality assurance of the NIH 3T3 cell line

Certificado de Análise (CoA)

Organismo	Rato
Tecido	Coração, miocárdio

Organismo	Rat
Tecido	Skeletal muscle