NCI-N87 Cells

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305057

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	NCI-N87, also known as N87, is a human gastric cancer cell line and is widely utilized in cancer research, particularly gastric carcinoma studies. NCI-N87 cells contribute to our understanding of the digestion model of the gastric mucosa and play a role in the development of gastroretentive delivery systems. In pharmacological contexts, NCI-N87 cells have been used to explore the role of gentamicin as an anticancer agent. The gastric adenocarcinoma cell line NCI-N87 is tumorigenic and expresses the oncogenes myc and erb-B2, and are therefore instrumental in xenograft model studies. This cell line's inflammatory properties and response to agents like gentamicin can be assayed, as can its potential involvement in epithelial barrier integrity and function using intestinal permeability assays. The cells are known to express surface glycoproteins such as carcinoembryonic antigen (CEA) and TAG 72, but are negative for L-dopa decarboxylase (DDC). The cells show minimal positivity for vasoactive intestinal peptide (VIP) receptors and lack gastrin receptors, and they express receptors for muscarinic cholinergic agents. No amplification or rearrangements were observed in N-myc, L-myc, myb, and EGF receptor genes in these cells. In summary, the gastric epithelium cell line NCI-N87 serves as a model for gastric cancer research, epithelial cell behavior, drug delivery systems, and the metabolic pathways of nutritionally relevant compounds.
Organismo	Human
Tecido	Stomach
Doença	Gastric tubular adenocarcinoma
Local da metástase	Liver
Sinônimos	NCI-N87, NCI N87, N-87, NCI-H87, H87, H-87, NCIN87

Gênero	Male
Etnia	African
Morfologia	Epithelial
Propriedades de crescimento	Adherent

Referência	NCI-N87 (Cytion catalog number 305057)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1603

Tumorigênico	Yes

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS, 10 mM HEPES, 2,5g/L Glucose and 1mM Sodiumpyruvate
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.