RBL-2H3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305194

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The RBL-2H3 cell line has become a valuable tool for studying mast cell physiology. RBL-2H3 cells express rat mast cell protease II (RMCP-II) and the c-kit receptor tyrosine kinase, making them a potential model for mast cells. However, conflicting and sometimes misleading data about RBL-2H3 cells have been reported. RBL-2H3 cells have been widely used to investigate various aspects of mast cell function, including degranulation, mast cell stabilizers, and the interaction of FceRI receptors with the cytoskeleton. They express high-affinity IgE receptors and can be activated to secrete histamine and other mediators. Cultivating RBL-2H3 cells is relatively easy, and longer culturing times result in higher cell density. Degranulation is a key feature of RBL-2H3 cells, similar to mast cells and basophils. When allergens crosslink their IgE-bound FceRI receptors, RBL-2H3 cells release preformed and newly synthesized mediators, contributing to immune allergic responses. The degranulation of RBL-2H3 cells has provided insights into basophil degranulation as well. These cells can also undergo degranulation in response to non-immunological stimuli, and there are differences between MMC, RBL-2H3, and CTMC. The role of calcium in RBL-2H3 cell degranulation is significant. The calcium ionophore A23187, which increases intracellular calcium levels, induces degranulation in RBL-2H3 cells, similar to mast cells and basophils. Some studies have described RBL-2H3 cells as a serotonin-releasing cell line.
Organismo	Rat
Tecido	Peripheral blood
Doença	Rat leukemia
Sinônimos	RBL2H3, RBL 2H3, RBL.2H3

Raça/Subespécie	Wistar
Morfologia	Fibroblast
Propriedades de crescimento	Adherent

Referência	RBL-2H3 (Cytion catalog number 305194)
Nível de biossegurança	1
NCBI_TaxID	10116
Número de acesso do Cellosaurus	CVCL_0591

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305194-041022	Certificado de Análise	23. May. 2025	305194
305194-120124	Certificado de Análise	23. May. 2025	305194
305194-300625	Certificado de Análise	22. Oct. 2025	305194

RBL-2H3 Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)